Laser-Induced Breakdown Spectroscopy in Vegetable Analysis: Contaminants and Nutrients

Authors

  • A. Anwar Laser Spectroscopy Lab., Department of Physics, University of Agriculture Faisalabad, 38040, Punjab, Pakistan
  • A. Rashid Department of Zoology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan
  • M. Rashid Department of Physics, University of Auckland, Private Bag 92019, Auckland, New Zealand

DOI:

https://doi.org/10.71330/thenucleus.2025.1479

Abstract

Vegetables are rich in minerals, but pollutants and wastewater, which introduce heavy metals into the soil, heavily impact their cultivation. As an efficient and effective methodology, scientists prefer using Laser-Induced Breakdown Spectroscopy (LIBS) as a light-based technique to determine the elemental constituents of vegetables. It aids in safety and quality assurance by allowing them to image nutrients and hazardous metals, such as cadmium. This study explored the application of LIBS for detecting contaminants, such as Cd, and profiling essential nutrients in vegetables. Unlike conventional methods such as ICP-MS and AAS, LIBS offers fast, on-site, and multi-element analyses with minimal sample preparation. This review consolidates recent studies on carrots, potatoes, spinach, broccoli, and other leafy greens, emphasizing enhancements using nanoparticles and chemometric tools to improve sensitivity and accuracy. According to the results, LIBS has also been effectively employed to analyze the components of vegetables, enhancing the control and safety of food quality surveillance. The results also prove that LIBS can be a better method for monitoring food quality and safety. LIBS is more consumer-and environmentally friendly because it is portable, fast, and capable of simultaneously analyzing various components.

References

N. Kaur, R. Singh, A. Sharma, P. Verma, and S. Gupta, "Heavy metal contamination in wastewater-irrigated vegetables: assessing food safety challenges in developing Asian countries," Environ. Sci.: Processes Impacts, vol. 27, pp. 1747–1767, 2025.

S. Shahriar, A. Rahman, M. Islam, R. Hasan, and T. Ahmed, "Heavy metal contamination in soil and vegetables: A review with health risk assessments," J. Sci. Eng. Papers, vol. 1, no. 1, pp. 40–48, 2024.

P. Kumari, V. Kachhwaha, and P. Mishra, "A comparative study of heavy metal toxicity in the vegetables using ICP-MS and AAS," Orient. J. Chem., vol. 40, no. 2, pp. 446–453, 2024.

Z. Yang, L. Huang, M. Chen, J. Li, and Y. Zhao, "Enhanced laser-induced breakdown spectroscopy for heavy metal detection in agriculture: A review," Sensors, vol. 22, no. 15, p. 5679, 2022.

X. Zhao, L. Chen, M. Wang, Y. Liu, and J. Zhang, "Detecting and mapping harmful chemicals in fruit and vegetables using nanoparticle-enhanced laser-induced breakdown spectroscopy," Sci. Rep., vol. 9, no. 1, p. 906, 2019.

F. Anabitarte, A. Cobo, and J. M. Lopez-Higuera, "Laser‐induced breakdown spectroscopy: fundamentals, applications, and challenges," Int. Scholarly Res. Notices, vol. 2012, pp. 1–12, 2012.

M. Gragston, L. Evans, J. Turner, S. Patel, and R. James, "Time-gated single-shot picosecond laser-induced breakdown spectroscopy (ps-LIBS) for equivalence-ratio measurements," Appl. Spectrosc., vol. 74, no. 3, pp. 340–346, 2020.

M. Gragston, L. Evans, J. Turner, S. Patel, and R. James, "Emissions in short-gated ns/ps/fs-LIBS for fuel-to-air ratio measurements in methane-air flames," Appl. Opt., vol. 60, no. 15, pp. C114–C120, 2021.

X. Liu, J. Zhang, L. Wang, Y. Chen, and H. Li, "Effect of laser pulse energy on orthogonal double femtosecond pulse laser-induced breakdown spectroscopy," Opt. Express, vol. 21, no. S4, pp. A704–A713, 2013.

Y. Zhang, X. Li, J. Chen, H. Wang, and L. Zhou, "Echelle grating spectroscopic technology for high-resolution and broadband spectral measurement," Appl. Sci., vol. 12, no. 21, p. 11042, 2022.

W. Wang, J. Liu, Y. Chen, X. Zhang, and L. Zhao, "Staging classification of omicron variant SARS-CoV-2 infection based on dual-spectrometer LIBS (DS-LIBS) combined with machine learning," Opt. Express, vol. 31, no. 25, pp. 42413–42427, 2023.

V. K. Singh, R. Kumar, S. Sharma, A. Gupta, and P. Verma, "Application of LIBS to elemental analysis and mapping of plant samples," At. Spectrosc., vol. 42, no. 1, pp. 446–453, 2021.

T. Shen, L. Zhou, Y. Wang, H. Liu, and J. Zhang, "High-sensitivity determination of nutrient elements in Panax notoginseng by laser-induced breakdown spectroscopy and chemometric methods," Molecules, vol. 24, no. 8, p. 1525, 2019.

G. Kim, J. Lee, S. Park, H. Choi, and M. Kang, "Detection of nutrient elements and contamination by pesticides in spinach and rice samples using laser-induced breakdown spectroscopy (LIBS)," J. Agric. Food Chem., vol. 60, no. 3, pp. 718–724, 2012.

M. Markiewicz-Keszycka, M. B. Kaczyński, K. Gajek, A. Woźniak, and A. Słowińska, "Laser-induced breakdown spectroscopy (LIBS) for food analysis: A review," Trends Food Sci. Technol., vol. 65, pp. 80–93, 2017.

I. Palamarchuk, M. Kovács, L. Novak, A. Horváth, and P. Varga, "Spectroscopic assessment and quantitative analysis of the trace element composition of vegetable additives to meat products," Slovak J. Food Sci., vol. 18, pp. 480–496, 2024.

Z. Chen, L. Wang, H. Liu, Y. Zhang, and X. Li, "Signal enhancement of cadmium in lettuce using laser-induced breakdown spectroscopy combined with pyrolysis process," Molecules, vol. 24, no. 13, p. 2517, 2019.

D. Stefas and N. Gyftokostas, "Laser-induced breakdown spectroscopy: An efficient tool for food science and technology (from the analysis of Martian rocks to the analysis of olive oil, honey, milk, and other natural earth products)," Molecules, vol. 26, no. 16, p. 4981, 2021.

D. M. Silvestre, L. A. Silva, M. R. Oliveira, P. J. Santos, and F. C. Almeida, "Direct analysis of barium, calcium, potassium, and manganese concentrations in tobacco by laser-induced breakdown spectroscopy," Microchem. J., vol. 126, pp. 545–550, 2016.

J. Molina M., L. Fernández, R. Gómez, A. Torres, and M. Sánchez, "Assessing the sensitivity and efficiency of laser-induced breakdown spectroscopy (LIBS) for high-concentration cadmium detection in cocoa powder," Sensors, vol. 25, no. 8, p. 2434, 2025.

C. R. Bhatt, S. Kumar, A. Singh, R. Sharma, and P. Verma, "Comparative study of elemental nutrients in organic and conventional vegetables using laser-induced breakdown spectroscopy (LIBS)," Appl. Spectrosc., vol. 71, no. 4, pp. 686–698, 2017.

Z. J. Kamil, M. J. Zoory, and H. J. Mohamad, "LIBS technique for plant mineral ratio analysis and environmental and agricultural importance: A comprehensive review," Eur. Phys. J. D, vol. 78, no. 3, p. 27, 2024.

S. A. Beldjilali, M. Abdou, A. Bendjeddou, and K. Boumendjel, "Analyses of plasmas produced by laser ablation of fresh aliments," Adv. Mater. Res., vol. 227, pp. 49–52, 2011.

N. Shukla, P. Kumar, R. Singh, A. Gupta, and S. Verma, "Determination of elements in carrot root by laser induced breakdown spectroscopy," Natl. Acad. Sci. Lett., vol. 40, pp. 47–51, 2017.

M. Yao, J. Chen, L. Wang, H. Zhao, and S. Li, "Detection of heavy metal Cd in polluted fresh leafy vegetables by laser-induced breakdown spectroscopy," Appl. Opt., vol. 56, no. 14, pp. 4070–4075, 2017.

N. Yudasari, S. Prasetyo, and M. Suliyanti, "The 1064 nm laser-induced breakdown spectroscopy (LIBS) inspection to detect the nutrient elements in freshly cut carrot samples," J. Phys.: Conf. Ser., vol. 985, no. 1, p. 012011, 2018.

P. Devangad, R. Sharma, S. Kulkarni, M. Singh, and A. Patel, "Plasma spectroscopy + chemometrics: An ideal approach for the spectrochemical analysis of iron phosphate glass samples," J. Chemometrics, vol. 34, no. 11, p. e3310, 2020.

B. L. Dutrow, N. J. McMillan, and D. J. Henry, "A multivariate statistical approach for mineral geographic provenance determination using laser-induced breakdown spectroscopy and electron microprobe chemical data: A case study of copper-bearing tourmalines," Am. Mineral., vol. 109, no. 6, pp. 1085–1095, 2024.

Y. Yuan, H. Liu, X. Li, J. Zhang, and M. Wang, "Improved discrimination for Brassica vegetables treated with agricultural fertilizers using a combined chemometric approach," J. Agric. Food Chem., vol. 64, no. 28, pp. 5633–5643, 2016.

O. Hamdy, Z. Abdel-Salam, and M. Abdel-Harith, "Utilization of laser-induced breakdown spectroscopy, with principal component analysis and artificial neural networks in revealing adulteration of similarly looking fish fillets," Appl. Opt., vol. 61, no. 34, pp. 10260–10266, 2022.

X. Wu, H. Li, Y. Chen, W. Zhang, and L. Wang, "Rapid food authentication using a portable laser-induced breakdown spectroscopy system," Foods, vol. 12, no. 2, pp. 1–18, 2023.

C. Liu, J. Zhang, L. Wang, Y. Chen, and H. Li, "Development and field tests of a deep-sea laser-induced breakdown spectroscopy (LIBS) system for solid sample analysis in seawater," Sensors, vol. 20, no. 24, p. 7341, 2020.

X. Fu, G. Li, and D. Dong, "Improving the detection sensitivity for laser-induced breakdown spectroscopy: A review," Front. Phys., vol. 8, p. 68, 2020.

C. Davison, T. Rodgers, M. Montes-Bayón, M. Thévenot, and P. J. Sadler, "Expanding the boundaries of atomic spectroscopy at the single-cell level: Critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells," Anal. Bioanal. Chem., vol. 415, no. 28, pp. 6931–6950, 2023.

L. Wang, Y. Zhang, H. Li, J. Chen, and M. Zhao, "Application and research progress of laser-induced breakdown spectroscopy in agricultural product inspection," ACS Omega, vol. 9, no. 23, pp. 24203–24218, 2024.

V. N. Lednev, A. S. Ivanov, M. P. Smirnov, and E. V. Kuznetsova, "Improving calibration strategy for LIBS heavy metals analysis in agriculture applications," Photonics, vol. 8, no. 12, p. 563, 2021.

S. Yao, M. Chen, L. Zhang, H. Liu, and Q. Wang, "Optimizing the binder percentage to reduce matrix effects for the LIBS analysis of carbon in coal," J. Anal. At. Spectrom., vol. 32, no. 4, pp. 766–772, 2017.

Y. Zhang, X. Li, J. Chen, H. Wang, and L. Zhou, "Pressure effects on underwater laser-induced breakdown spectroscopy: An interpretation with self-absorption," J. Anal. At. Spectrom., vol. 36, no. 3, pp. 644–653, 2021.

A. J. Effenberger, Jr. and J. R. Scott, "Effect of atmospheric conditions on LIBS spectra," Sensors, vol. 10, no. 5, pp. 4907–4925, 2010.

R. C. Madasani and M. Hossain, "Impact of surface texture on moisture absorption and long-term mechanical performance of biomedical polymers," in Proc. ASME Int. Mech. Eng. Congr. Expo., vol. 4, Portland, OR, USA, Nov. 2024.

E. Böhmer, M. Scholz, F. Müller, T. Schneider, and A. Becker, "Preliminary results for calibration-free laser-induced breakdown spectroscopy (CF-LIBS) for the elemental analysis of biological samples," in Optical Interactions with Tissue and Cells XXXVI, SPIE, vol. 13317, p. 1331706, 2025.

R. R. Gamela, A. M. G. T. da Silva, M. A. P. Soares, J. A. S. Cavichio, and J. L. F. Monteiro, "Matrix-matching calibration using solid standards: A comparison between univariate and multivariate strategies for the determination of calcium and magnesium in bean seed samples employing laser-induced breakdown spectroscopy (LIBS)," Anal. Lett., vol. 56, no. 6, pp. 944–957, 2023.

M. Rashid, A. Ahmed, S. Khan, F. Ali, and Z. Malik, "Laser-induced breakdown spectroscopy for soil analysis: Recent advances in nutrient and contaminant detection," Spectrum of Eng. Sci., vol. 3, no. 8, pp. 279–288, 2025.

L. Na, E. Harefa, and Z. Weidong, "Nanosecond laser preheating effect on ablation morphology and plasma emission in collinear dual-pulse laser-induced breakdown spectroscopy," Plasma Sci. Technol., vol. 24, no. 11, p. 115507, 2022.

A. Erler, M. Thiele, H. Küpper, T. Schmid, and H. Knicker, "Soil nutrient detection for precision agriculture using handheld laser-induced breakdown spectroscopy (LIBS) and multivariate regression methods (PLSR, Lasso and GPR)," Sensors, vol. 20, no. 2, p. 418, 2020.

M. Wójcik, J. Baranowski, M. Kwiatkowski, M. Dudzik, and K. Błaszkiewicz, "Classification of copper minerals by handheld laser-induced breakdown spectroscopy and nonnegative tensor factorisation," Sensors, vol. 20, no. 18, p. 5152, 2020.

T. Wu, R. Zhai, J. Huang, Z. Wang, B. Han, and Y. Liu, "Deep learning-enhanced laser-induced breakdown spectroscopy for rapid in-situ analysis of Martian surface and atmospheric constituents," Microwave Opt. Technol. Lett., vol. 67, no. 6, p. e70273, 2025.

R. Gaudiuso, A. Taleb, M. Dell'Aglio, I. C. Tommasi, and A. De Giacomo, "Feasibility of nanoparticle-enhanced LIBS (NELIBS) for the analysis of archaeological metallic artifacts: A critical assessment," J. Anal. At. Spectrom., vol. 40, no. 2, pp. 354–364, 2025.

P. Mahyari, M. Maniscalco, H. Choi, N. May, A. Phoulady, T. Moore,

A. Blagojevic, M. T. M. Anaei, T. Bliznakov, M. Emanuel, W. Roser, S. Shahbazmohamadi, and P. Tavousi, "Laser-induced breakdown spectroscopy for real-time 3D material composition mapping," in Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XXV, SPIE, vol. 13353, pp. 58–69, 2025.

X. Wu, S. Shin, E. Bae, J. P. Robinson, and B. Rajwa, "Food authentication studies using laser-induced breakdown spectroscopy (LIBS)," in Sensing for Agriculture and Food Quality and Safety XIV, SPIE, vol. 12120, pp. 76–83, 2022.

Downloads

Published

15-09-2025

How to Cite

[1]
H. A. Anwar, A. Rashid, and R. Muhammad, “Laser-Induced Breakdown Spectroscopy in Vegetable Analysis: Contaminants and Nutrients”, The Nucleus, vol. 62, no. 2, pp. 69–75, Sep. 2025.

Issue

Section

Articles